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metadata.dc.type: | Artigo de Periódico |
Title: | Macrophages participate in host protection and the disease pathology associated with Leishmania braziliensis infection |
Other Titles: | BMC Infectious Diseases |
Authors: | Giudice, Angela Vendrame, Célia Bezerra, Caroline A. Carvalho, Lucas Pedreira de Delavechia, Thaís Carvalho Filho, Edgar Marcelino de Bacellar, Maria Olívia Amado Ramos |
metadata.dc.creator: | Giudice, Angela Vendrame, Célia Bezerra, Caroline A. Carvalho, Lucas Pedreira de Delavechia, Thaís Carvalho Filho, Edgar Marcelino de Bacellar, Maria Olívia Amado Ramos |
Abstract: | Background Leishmania preferentially infects macrophages, which allow the parasite to multiply but can also kill the parasite. Although the T cell response in human leishmaniasis is well-characterized, little is known about the concomitant macrophage behavior. The aim of this study was to characterize the macrophage immune response after Leishmania braziliensis infection in cells derived from cutaneous leishmaniasis (CL) or mucosal leishmaniasis (ML) patients, subclinical individuals (SC) and healthy control subjects (HS). Methods Peripheral blood mononuclear cell-derived macrophages from the different groups were exposed to L. braziliensis in vitro and were evaluated for susceptibility to Leishmania infection, ability to kill Leishmania and chemokine/cytokine production. Nitric Oxide (NO) and superoxide (O2-) levels in the supernatant of infected macrophage cultures were monitored. Results After exposure to L. braziliensis, peripheral blood mononuclear cell-derived macrophages from SC individuals showed a lower infection rate and a smaller number of intracellular amastigotes compared to cells from CL and ML patients. Macrophages from CL and ML patients produced more chemokines and TNF-α than those from the SC group. Production of NO and O2- were detected but did not vary significantly among the different groups. Conclusions Our data indicate that macrophages play a pivotal role in controlling L. braziliensis infection and in leishmaniasis pathology by secreting pro-inflammatory chemokines/cytokines that activate and recruit T cells, overwhelming the inflammatory response. |
Keywords: | L braziliensis Macrophages Chemokines Subclinical infection |
metadata.dc.rights: | Acesso Aberto |
URI: | http://repositorio.ufba.br/ri/handle/ri/16189 |
Issue Date: | 2012 |
Appears in Collections: | Artigo Publicado em Periódico (Faculdade de Medicina) |
Files in This Item:
File | Description | Size | Format | |
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Angela Giudice.pdf | 539,15 kB | Adobe PDF | View/Open |
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