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https://repositorio.ufba.br/handle/ri/7764
metadata.dc.type: | Artigo de Periódico |
Título : | An improved anti-C3/IgG ELISA for quantification of soluble immune complexes |
Otros títulos : | Journal of Immunological Methods |
Autor : | Soares, Neci Matos Santiago, Mittermayer Barreto Carvalho, Lain Carlos Pontes de |
metadata.dc.creator: | Soares, Neci Matos Santiago, Mittermayer Barreto Carvalho, Lain Carlos Pontes de |
Resumen : | A semi-quantitative ELISA for complement-fixing, IgG-containing immune complexes (IC) is described. The assay is based on the insolubilization of IC by polyethyleneglycol, their capture by solid-phase anti-C3 antibodies, reaction with peroxidase-labeled anti-IgG antibodies and incubation with a chromogenic peroxidase substrate. It was markedly improved by the use of a single-step procedure which simultaneously washed and precipitated the insolubilized immune complexes. Intra-assay and inter-assay coefficients of variation were lower than 8.6 and 14.7%, respectively. As expected, higher levels of circulating immune complexes, in relation to healthy individuals, were found in patients with American visceral leishmaniasis (AVL), systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA), with prevalences comparable to those described in the literature. The ELISA can be quickly assembled from reagents and plasticware widely available commercially, detects immune complexes fulfilling three different criteria and is more sensitive than a previously published method based on the same principles (detection limit for complement-sensitized aggregated IgG of 2 μg ml−1 as compared with a detection limit above 16 μg ml−1). |
Palabras clave : | Immune complexes Polyethylene glycol ELISA C3 IgG |
URI : | http://www.repositorio.ufba.br/ri/handle/ri/7764 |
Fecha de publicación : | 2001 |
Aparece en las colecciones: | Artigo Publicado em Periódico (FAR) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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Soares.pdf Restricted Access | 187,29 kB | Adobe PDF | Visualizar/Abrir Request a copy |
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