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metadata.dc.type: | Artigo de Periódico |
Title: | Comparison of three decontamination methods for Mycobacterium bovis isolation |
Other Titles: | Brazilian Journal of Microbiology |
Authors: | Ambrosio, Simone Rodriguez Oliveira, Eugênia Márcia de Deus Rosales Rodriguez, Cesar Alejandro Ferreira Neto, José Soares Amaku, Marcos |
metadata.dc.creator: | Ambrosio, Simone Rodriguez Oliveira, Eugênia Márcia de Deus Rosales Rodriguez, Cesar Alejandro Ferreira Neto, José Soares Amaku, Marcos |
Abstract: | Sixty samples of tissue fragments with lesions suggestive of tuberculosis from bovine abattoirs, kept in saturated solution of sodium borate, were subjected to four treatments: 4% NaOH (Petroff Method), 12 % H2SO4 and 1.5% HPC (1-Hexadecylpyridinium Chloride) decontamination, and physiological saline solution (control). The HPC method showed the lowest contamination rate (3%) when compared to control (88%, p<0.001), NaOH (33%, p<0.001) and H2SO4 (21.7%, p<0.002). Regarding the isolation success, the HPC method was better (40%) than the control (3%, p<0.001), NaOH (13%, p=0.001) and H2SO4 (1.7%, p<0.001) methods. These results indicate that HPC is an alternative to the Petroff method. |
Keywords: | Mycobacterium bovis Decontamination Petroff HPC |
Publisher: | Sociedade Brasileira de Microbiologia |
URI: | http://www.repositorio.ufba.br/ri/handle/ri/6692 |
Issue Date: | Apr-2008 |
Appears in Collections: | Artigo Publicado em Periódico (EMV) |
Files in This Item:
File | Description | Size | Format | |
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Ambrosio, Simone Rodrigues.pdf | 381,81 kB | Adobe PDF | View/Open |
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