Please use this identifier to cite or link to this item:
https://repositorio.ufba.br/handle/ri/6257
metadata.dc.type: | Artigo de Periódico |
Title: | Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva |
Other Titles: | BMC Immunology |
Authors: | Menezes, Maria José Costa, Dirceu Joaquim Clarêncio, Jorge Miranda, José Carlos Barral, Aldina Maria Prado Barral-Netto, Manoel Brodskyn, Claudia Ida Oliveira, Camila Indiani de |
metadata.dc.creator: | Menezes, Maria José Costa, Dirceu Joaquim Clarêncio, Jorge Miranda, José Carlos Barral, Aldina Maria Prado Barral-Netto, Manoel Brodskyn, Claudia Ida Oliveira, Camila Indiani de |
Abstract: | Background: Sand fly saliva contains potent and complex pharmacologic molecules that are able to modulate the host's hemostatic, inflammatory, and immune systems. In this study, we evaluated the effects of salivary gland sonicate (SGS) of Lutzomyia intermedia, the natural vector of Leishmania braziliensis, on monocytes obtained from the peripheral blood mononuclear cells (PBMC) of healthy volunteers. We investigated the effects of sand fly saliva on cytokine production and surface molecule expression of LPS-stimulated human monocytes uninfected or infected with L. braziliensis. Results: Pre-treatment of non-infected human monocytes with L. intermedia SGS followed by LPSstimulation led to a significant decrease in IL-10 production accompanied by a significant increase in CD86, CD80, and HLA-DR expression. Pre-treatment with SGS followed by LPS stimulation and L. braziliensis infection led to a significant increase in TNF-α, IL-6, and IL-8 production without significant alterations in co-stimulatory molecule expression. However, pre-treatment with L. intermedia SGS did not result in significant changes in the infection rate of human monocytes. Conclusion: Our data indicate that L. intermedia saliva is able to modulate monocyte response, and, although this modulation is dissociated from enhanced infection with L. braziliensis, it may be associated with successful parasitism. |
URI: | http://www.repositorio.ufba.br/ri/handle/ri/6257 |
Issue Date: | 2008 |
Appears in Collections: | Artigo Publicado em Periódico (Faculdade de Medicina) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
C__Documents and Settings_rep...t.default_Cache_3_8C_459AEd01.pdf | 365,49 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.