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https://repositorio.ufba.br/handle/ri/12857
metadata.dc.type: | Artigo de Periódico |
Título : | Effects of the flavonoid casticin from Brazilian Croton betulaster in cerebral cortical progenitors in vitro: direct and indirect action through astrocytes |
Otros títulos : | Journal of Neuroscience Research |
Autor : | Spohr, Tânia Cristina Leite de Sampaio e Stipursky, Joice Sasaki, Adriana Campos Barbosa, Pedro Rocha Martins, Vanessa Benjamim, Cláudia Farias Roque, Nídia Franca Costa, Silvia Lima Gomes, Flávia Carvalho Alcantara |
metadata.dc.creator: | Spohr, Tânia Cristina Leite de Sampaio e Stipursky, Joice Sasaki, Adriana Campos Barbosa, Pedro Rocha Martins, Vanessa Benjamim, Cláudia Farias Roque, Nídia Franca Costa, Silvia Lima Gomes, Flávia Carvalho Alcantara |
Resumen : | Neurodegenerative diseases are a major constraint on the social and economic development of many countries. Evidence has suggested that phytochemicals have an impact on brain pathology; however, both their mechanisms of action and their cell targets are incompletely known. Here, we investigated the effects of the flavonoid casticin, extracted from Croton betulaster, a common plant in the state of Bahia in Brazil, on rat cerebral cortex neurons in vitro. Treatment of neural progenitors with 10 μM casticin increased the neuronal population positive for the neuronal marker β-tubulin III and the neuronal transcriptional factor Tbr2 by approximately 20%. This event was followed by a 50% decrease in neuronal death. Pools of astrocyte (GFAP and S100β), neural (nestin), and oligodendrocyte (Olig2 and NG2) progenitors were not affected by casticin. Neither neuronal commitment nor proliferation of progenitors was affected by casticin, suggesting a neuroprotective effect of this compound. Culture of neural progenitors on casticin-treated astrocyte monolayers increased the neuronal population by 40%. This effect was reproduced by conditioned medium derived from casticin-treated astrocytes, suggesting the involvement of a soluble factor. ELISA assays of the conditioned medium revealed a 20% increase in interleukin-6 level in response to casticin. In contrast to the direct effect, neuronal death was unaffected, but a 52% decrease in the death of nestin-positive progenitors was observed. Together our data suggest that casticin influences the neuronal population by two mechanisms: 1) directly, by decreasing neuronal death, and 2) indirectly, via astrocytes, by modulating the pool of neuronal progenitors. © 2009 Wiley-Liss, Inc. |
Palabras clave : | Neuron–glia interaction Flavonoids Astrocyte Neurodegenerative diseases Neurogenesis |
URI : | http://www.repositorio.ufba.br/ri/handle/ri/12857 |
Fecha de publicación : | 2010 |
Aparece en las colecciones: | Artigo Publicado em Periódico (ICS) |
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