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metadata.dc.type: | Artigo de Periódico |
Title: | IL-10 and IL-12 are the main regulatory cytokines in visceral leishmaniasis |
Other Titles: | Cytokine |
Authors: | Bacellar, Maria Olívia Amado Ramos D'Oliveira Junior, Argemiro Jerônimo, Selma Carvalho Filho, Edgar Marcelino de |
metadata.dc.creator: | Bacellar, Maria Olívia Amado Ramos D'Oliveira Junior, Argemiro Jerônimo, Selma Carvalho Filho, Edgar Marcelino de |
Abstract: | Visceral leishmaniasis (VL) is characterized by the absence of cytokines such as IFN-γ and IL-12. Cure of VL is associated with a restoration of the ability to make these cytokines. The aim of the present study was to evaluate the role of IL-12 in the recovery of the ability to produce IFN-γ and to test whether or not IL-4 IL-10 and/or TGF-β could suppress IFN-γ production by PBMC from treated VL patients. High stimulation index (SI) of proliferation was observed in PBMC from subjects stimulated with Leishmania chagasi antigen (181±83). Neutralizing IL-12 inhibited lymphoproliferation [stimulation index (SI) of 210±114 to 1±0.6 (P<0.01)] and/or the production of IFN-γ [2792±402 pg/ml to 407±449 pg/ml (P<0.01)]. Recombinant IL-10 abrogated the lymphoproliferation (SI=2±3) while recombinant IL-4 or TGF-β had no effect on this response (147±22 and 194±12 respectively). IFN-γ was high when PBMCs were stimulated with L. chagasi (873±400 pg/ml) and this was abrogated by the addition of IL-10 (5±2 pg/ml). In contrast neither IL-4 or TGF-β suppressed IFN-γ production (837±244 pg/ml and 759±523 pg/ml). These results indicate that IL-12 plays an important role in the ability of treated VL patients to make IFN-γ and that IL-10 but not IL-4 or TGF-β inhibits this response. |
Keywords: | cytokines interleukin 10 interleukin 12 leishmaniasis visceral leishmaniasis |
URI: | http://www.repositorio.ufba.br/ri/handle/ri/7814 |
Issue Date: | 2000 |
Appears in Collections: | Artigo Publicado em Periódico (Biologia) |
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1-s2.0-S104346660090694X-main.pdf Restricted Access | 91,5 kB | Adobe PDF | View/Open Request a copy |
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