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https://repositorio.ufba.br/handle/ri/6607
metadata.dc.type: | Artigo de Periódico |
Title: | Human T cell epitope mapping of the Schistosoma mansoni 14-kDa fatty acid-binding protein using cells from patients living in areas endemic for schistosomiasis |
Other Titles: | Microbes and Infection |
Authors: | Fonseca, Cristina Toscano Cunha Neto, Edécio Goldberg, Anna Carla Renata Krepel Kalil, Jorge Jesus, Amélia Maria Ribeiro de Carvalho Filho, Edgar Marcelino de Oliveira, Rodrigo Correa Oliveira, Sérgio Costa |
metadata.dc.creator: | Fonseca, Cristina Toscano Cunha Neto, Edécio Goldberg, Anna Carla Renata Krepel Kalil, Jorge Jesus, Amélia Maria Ribeiro de Carvalho Filho, Edgar Marcelino de Oliveira, Rodrigo Correa Oliveira, Sérgio Costa |
Abstract: | he development of a defined anti-schistosomiasis vaccine would contribute to the current control strategy mainly because immunization provides long-lasting immunity to the disease. Sm14, one of the six Schistosoma mansoni antigens selected by WHO as a candidate to compose a subunit vaccine against schistosomiasis, has been associated with resistance to S. mansoni infection in human beings and is able to induce protection in the murine model. To identify human T cell epitopes in Sm14, we used the TEPITOPE algorithm to select peptides that would most likely bind to several HLA-DR molecules. In this study, three Sm14 epitopes were selected and produced as synthetic peptides. Human T cell responses from schistosomiasis patients living in endemic areas in Brazil were determined by proliferation assay and IL-5 and IFN-γ measurements. Differential peptide recognition and cytokine production in response to Sm14 epitopes were observed in individuals resistant to S. mansoni infection versus susceptible individuals. Sm14(32-48) and Sm14(53-69) peptides were preferentially recognized by peripheral blood mononuclear cells (PBMCs) of S. mansoni-resistant individuals, and Sm14(53-69) induced significant production of IFN-γ. Additionally, Sm14(32-48) and Sm14(53-69) were “promiscuous” peptides, since they were able to induce cellular immune responses in individuals carrying 10 and 8, respectively, of the 11 HLA-DR molecules expressed in the studied population. Among Sm14 synthetic peptides tested in this study, we identified Sm14(32-48) and Sm14(53-69) as promising candidates to compose an anti-schistosomiasis vaccine, since they seem to be related to resistance to human schistosomiasis. |
Keywords: | Vaccine Human T cells Synthetic peptides Schistosoma mansoni Sm14 |
Publisher: | Elsevier |
URI: | http://www.repositorio.ufba.br/ri/handle/ri/6607 |
Issue Date: | Feb-2005 |
Appears in Collections: | Artigo Publicado em Periódico (Faculdade de Medicina) |
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