Por favor, use este identificador para citar o enlazar este ítem:
https://repositorio.ufba.br/handle/ri/5366
metadata.dc.type: | Artigo de Periódico |
Título : | A piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum |
Otros títulos : | Analytical and Bioanalytical Chemistry |
Autor : | Jesus, Joilson Ramos Carvalho, Kellyanne dos Anjos Fonseca, Rosana Anita da Silva Oliveira, Geraldo Gileno de Sá Melo, Stella Maria Barrouin Neves, Neuza Maria Alcântara Dutra, Rosa Amália Fireman |
metadata.dc.creator: | Jesus, Joilson Ramos Carvalho, Kellyanne dos Anjos Fonseca, Rosana Anita da Silva Oliveira, Geraldo Gileno de Sá Melo, Stella Maria Barrouin Neves, Neuza Maria Alcântara Dutra, Rosa Amália Fireman |
Resumen : | The American visceral leishmaniasis is an important cause of morbidity and mortality in Brazil for both humans and dogs. Attempts to make a diagnosis of this disease need to be improved, especially in endemic areas, and in the tracking and screening of asymptomatic dogs, which are their main host in urban areas. A quartz crystal microbalance immunosensor for the diagnosis of the canine visceral leishmaniasis using a recombinant antigen of Leishmania chagasi (rLci2B-NH6) was devel- oped. The rLci2B-NH6 was tightly immobilized on a quartz crystal gold electrode by self-assembled monolayer based on short-chain length thiol. The strategy was the use of the antigen-histidine tail covalently linked to glutaral-dehyde performing a Schift base which permits a major exposure of epitopes and a reduced steric hindrance. The immunosensor showed good results regarding sensitivity and reproducibility, being able to distinguish positive and negative canine serum for L. chagasi. Furthermore, the immunosensor can be reused through exposure to sodium dodecyl sulfate solution, which promotes the dissociation of antigen–antibody binding, restoring the sensor surface with immobilized biologically active antigens for further analysis. |
Palabras clave : | Leishmania chagasi Immunosensor Self-assembled monolayer Piezoelectric |
URI : | http://www.repositorio.ufba.br/ri/handle/ri/5366 |
Fecha de publicación : | 2011 |
Aparece en las colecciones: | Artigo Publicado em Periódico (EMV) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
8951fulltext.pdf Restricted Access | 235,52 kB | Adobe PDF | Visualizar/Abrir Request a copy |
Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.