https://repositorio.ufba.br/handle/ri/17049 2026-04-17T09:15:35Z https://repositorio.ufba.br/handle/ri/17096 Título: Stress proteins accumulation in corbicula fluminea (Mollusca; Bivalvia) As an indication of environmental water quality related to heat shock protein respoinse Autor(es): Nascimento, Iracema Andrade Tipo: Artigo de Periódico 1996-01-01T00:00:00Z https://repositorio.ufba.br/handle/ri/17095 Título: Ecotoxicological diagnosis of Aratu Bay, Bahia, Brazil: a new approach to validate a reactive short-term toxicity end-point by comparison with intertidal benthic activity Autor(es): Nascimento, Iracema Andrade; Smith, D. H.; Gomes, Maria da Gloria Sampaio; Santos, Gislaine Vieira dos; Pereira, Solange Andrade Abstract: The Aratu Bay ecosystem (12°48′S; 38°28′W), located in an industrialized area, was compared with a similar but undisturbed ecosystem, Iguape Bay, in order to determine the impact of industry on water quality during the last forty years. Comparison was based on simple and low cost methodologies using bioassays on oyster embryos, validated by a survey of a benthic activity index. A bioassay method based on oyster embryo development was used to compare water quality from the study areas. A high percentage (93.2 and 97.3) of abnormal oyster embryo development was observed in water samples from one area (south and southeast) of the bay where, besides receiving heavy discharges of industrial effluents, water circulation is restricted. At the north end of the bay where the area is shallow, and some industrial discharges are present, water quality data indicated that the area was stressed. Little effect of industrial impact has been noted around the Cotegipe channel, a deep area where the water circulation pattern is heavy and the water well mixed. Validation of this survey has been achieved by the simple method of investigating benthic activity in the area. Quadrat counts of active benthic galleries were used as an index of community activity for comparative analysis between sampling stations and for discriminating between polluted and unpolluted sites. This research has shown that the quadrat counts of active benthic galleries, at least in areas of comparable homogeneous substrate, can be used as a viable index of environmental impact, when interpreted together with other source of data, such as early-life-stage tests. This new approach proved to be effective and led to the recommendation of remedial policies in the area. Tipo: Artigo de Periódico 2000-01-01T00:00:00Z https://repositorio.ufba.br/handle/ri/17094 Título: Integration of varying responses of different organisms to water and sediment quality at sites impacted and not impacted by the petroleum industry Autor(es): Nascimento, Iracema Andrade; Smith, D. H.; Pereira, Solange Andrade; Araujo, Milena Maria Sampaio de; Silva, M. A.; Mariani, A. M. Tipo: Artigo de Periódico 2000-01-01T00:00:00Z https://repositorio.ufba.br/handle/ri/17093 Título: Toxic Effects of Cryoprotectants on oyster gametes and embryos: A preliminary step towards establishing cryopreservation protocols Autor(es): Sansone, Giovanni; Nascimento, Iracema Andrade; Leite, Maria Bernadete Neiva Lemos; Araujo, Milena Maria Sampaio de; Pereira, Solange Andrade; Mariani, Ana Maria Abstract: Aquaculture development is dependent on continuous seed production, regardless of the spawning season. Cryopreservation can therefore, be a valuable tool for achieving seed availability. The benefits of cryopreservation have been demonstrated for mammals, fish and some achievements have been reported for invertebrates; however, cryopreservation has not yet contributed significantly to oyster rearing. The literature on this topic demonstrates that an optimal cryopreservation method developed for one species is not always applicable to another. One of the reasons is that there are differences among species in the toxicological responses of gametes and embryos to cryoprotectants. The aim of this study was to determine the toxic effects of cryoprotectants dimethylsulfoxide (Me2SO), glycerol (G), ethylene glycerol (EG), propylene glycerol (PG) and methanol (MET) on Crassostrea rhizophorae gametes and embryos). Gametes (oocytes and spermatozoa) and embryos (trochophores) were exposed to a range of concentrations of each cryoprotectant for 10, 20 and 30 minutes. Based on the EC50-24h values there were no significant differences (p > 0.05) among the exposure times in toxic effects to either gametes or embryos. The trochophores were relatively resistant to cryoprotectant exposure, while oyster gametes became increasingly susceptible to the cryoprotectants as concentration levels were increased. Critical values (EC50-24h) of cryoprotectants were markedly different for gametes and embryos. For gametes, both G and MET were more toxic (EC50-24h respectively of 3.46 and 4.52% for oocytes, and 2.07 and 11.21% for spermatozoa) than EG, PG and Me2SO. However, PG (EC50-24h = 23.56%) and EG (EC50-24h = 45.18%) were more toxic for trochophores than Me2SO (EC50-24h = 54.25) or MET(EC50-24h = 55.63%). These results show the importance of previous toxicological studies for cryoprotectant selection as a preliminary step towards establishing cryopreservation protocols. Tipo: Artigo de Periódico 2005-01-01T00:00:00Z