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https://repositorio.ufba.br/handle/ri/6268
metadata.dc.type: | Artigo de Periódico |
Título : | Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermia |
Otros títulos : | Experimental Neurology |
Autor : | Capani, Francisco Saraceno, Gustavo Ezequiel Botti, Valeria Aon Bertolino, Laura Oliveira, Diêgo Madureira de Barreto, George Emílio Sampaio Galeano, Pablo Giraldez Alvarez, Lisandro Diego Coirini, Héctor |
metadata.dc.creator: | Capani, Francisco Saraceno, Gustavo Ezequiel Botti, Valeria Aon Bertolino, Laura Oliveira, Diêgo Madureira de Barreto, George Emílio Sampaio Galeano, Pablo Giraldez Alvarez, Lisandro Diego Coirini, Héctor |
Resumen : | Synaptic dysfunction has been associated with neuronal cell death following hypoxia. The lack of knowledge on the mechanisms underlying this dysfunction prompted us to investigate the morphological changes in the postsynaptic densities (PSDs) induced by hypoxia. The results presented here demonstrate that PSDs of the rat neostriatum are highly modified and ubiquitinated 6 months after induction of hypoxia in a model of perinatal asphyxia. Using both two dimensional (2D) and three dimensional (3D) electron microscopic analyses of synapses stained with ethanolic phosphotungstic acid (E-PTA), we observed an increment of PSD thickness dependent on the duration and severity of the hypoxic insult. The PSDs showed clear signs of damage and intense staining for ubiquitin. These morphological and molecular changes were effectively blocked by hypothermia treatment, one of the most effective strategies for hypoxia-induced brain injury available today. Our data suggest that synaptic dysfunction following hypoxia may be caused by long-term misfolding and aggregation of proteins in the PSD. |
Palabras clave : | Postsynaptic density Hypoxia Neostriatum Ubiquitin Hypothermia Neuroprotection Perinatal asphyxia |
Editorial : | Elsevier |
URI : | http://www.repositorio.ufba.br/ri/handle/ri/6268 |
Fecha de publicación : | oct-2009 |
Aparece en las colecciones: | Artigo Publicado em Periódico (Biologia) |
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Fichero | Descripción | Tamaño | Formato | |
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(91)1-s2.0-S0014488609002362-main.pdf Restricted Access | 4,88 MB | Adobe PDF | Visualizar/Abrir Request a copy |
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